Gene expression analysis of JAGGED-1 treated human periodontal ligament stem cells using microarray technique

The present study aimed to evaluate mRNA expression profiling of Jagged1 treated human periodontal ligament cells (hPDLs) using microarray technique. Jagged1 regulates various biological pathway in hPDLs. Notch ligands, Jagged1, were indirectly immobilized on tissue culture surface. Subsequently, hPDLs were seeded on Jagged1 immobilized surface and maintained in growth medium for 48 h. Total RNA was collected and processed. Gene expression profiling was examined using microarray technique.

本研究旨在通过微阵列（microarray）技术，分析经Jagged1处理的人牙周膜细胞（human periodontal ligament cells，hPDLs）的mRNA表达谱。Jagged1可调控人牙周膜细胞内的多种生物学通路。实验中先将Notch配体Jagged1间接固定于组织培养表面，随后将人牙周膜细胞接种于该Jagged1固定化表面，并在生长培养基中培养48小时。收集总RNA并完成处理后，通过微阵列技术检测基因表达谱。