ChIP-sequencing analysis of RNA Pol II binding sites 4 hours after Myc activation in liver and heart tissue, from wild type and R26CAG-c-MycERT2 mice. ChIP-sequencing analysis of RNA Pol II binding sites 4 hours after Myc activation in liver and heart tissue, from wild type and R26CAG-c-MycERT2 mice

Aim: To determine RNA Pol II binding sites in mouse heart and liver, 4 hours post MycER activation. Pol II ChIP sequencing performed on chromatin isolated from hearts and livers harvested from wild-type (R26+/+) and R26CAG-c-MycERT2/+ mice 4 hours post administration of (Z)-4-hydroxytamoxifen.

研究目的：鉴定MycER激活4小时后小鼠心脏与肝脏中的RNA聚合酶II（RNA Polymerase II）结合位点。本实验对给予(Z)-4-羟基他莫昔芬4小时后处死的野生型（R26+/+）及R26CAG-c-MycERT2/+小鼠的心脏与肝脏组织分离得到的染色质，开展Pol II染色质免疫共沉淀测序（ChIP sequencing）。