Gene expression analysis in normal and regenerating liver from lean mice and mice with diet induced obesity. Mus musculus

Analysis of gene expression after sham surgery, 70% hepatectomy or 80% hepatectomy in normal mice or mice made obese through feeding of a high-fat diet. Overall design: Diet-induced obese (DIO) and lean control male C57BL/6J mice were obtained from Jackson Laboratories and maintained in our colony by feeding of the same dietary forumlation used by Jackson Labs. After several weeks of acclimation, mice were subjected to sham surgery (midline incision and liver manipulation only), 70% hepatectomy or 80% hepatectomy. Mice were euthanized under isoflurane anesthesia 6 hours later. Total RNA was isolated from snap frozen liver and subjected to Illumina gene expression analysis.

本数据集针对正常小鼠或经高脂饮食（high-fat diet）诱导的肥胖小鼠，分析其在假手术（sham surgery）、70%肝切除术或80%肝切除术后的基因表达情况。
实验设计概述：购自杰克逊实验室（Jackson Laboratories）的雄性C57BL/6J小鼠，分为饮食诱导肥胖（Diet-induced obese, DIO）组与瘦型对照组，使用与杰克逊实验室一致的饲料配方在本实验室种群中饲养。经过数周适应期后，对各组小鼠分别实施假手术（仅行正中切口与肝脏操作）、70%肝切除术或80%肝切除术。术后6小时，在异氟烷（isoflurane）麻醉下处死小鼠。从快速冷冻的肝脏组织中分离总RNA，并开展Illumina基因表达分析。