cryoEM-Montpellier

The montpellier’s cryoEM community and the requested BAG includes the groups from CBS, the IGF, of the IRIM and the LPHI. We are currently running several research projects requiring single-particles cryoEM data collection. We have both internal projects and long-term collaborative projects that currently relies on access to high-end 200KeV microscopes for sample screening mainly (METI Toulouse, Marseille, IBS Grenoble, Karolinska Institute) and to 300KeV electron microscopes for data collection ( ESRF running proposals, Karolinska institute, MRC-LMB, NCBS Bangalore, Thermofisher). In this context, we investigate the structural basis of important therapeutic targets. The CBS teams characterises nuclear hormone receptors, nucleotide binding proteins, enzymes, and DNA ORIGAMI. The IGF characterize GPCRs such as mGluRs, and related transmembrane proteins. The IRIM and LPHI are focusing on several therapeutic targets, including membrane proteins.

蒙彼利埃的冷冻电镜（cryoEM）研究社群与所申请的BAG项目，涵盖了来自CBS、IGF、IRIM及LPHI的研究团队。我们目前正在推进多项需开展单颗粒冷冻电镜数据采集的研究项目，包含内部项目与长期合作项目两类。当前，我们主要依托高端200keV电子显微镜开展样品筛选工作，合作单位包括图卢兹METI、马赛研究所、格勒诺布尔IBS以及卡罗林斯卡学院；同时使用300keV电子显微镜进行数据采集，合作方涵盖ESRF在研项目、卡罗林斯卡学院、MRC-LMB、班加罗尔NCBS以及赛默飞世尔科技（Thermofisher）。在此研究背景下，我们正致力于探究重要治疗靶点的结构基础。其中，CBS团队的研究对象包括核激素受体、核苷酸结合蛋白、酶以及DNA折纸（DNA ORIGAMI）；IGF团队聚焦于G蛋白偶联受体（GPCRs），例如代谢型谷氨酸受体mGluRs及其相关跨膜蛋白；IRIM与LPHI团队则针对包括膜蛋白在内的多种治疗靶点展开研究。