Zebrafish EN/ENCDC RNA-seq. Danio rerio

A project to find differential expressed genes between enteric neurons (ENs) and enteric neural crest-derived cells (ENCDCs) in larval zebrafish gut. We dissected guts of transgenic line, Tg(SAGFF(LF)217B; uas:gfp) for ENs and Tg(sox10:cre; EF1alpha:loxP-gfp-loxP-dsred) for ENCDCs, and isolated GFP+ ENs and dsRed+ ENCDCs. Three duplicates for each of ENs and ENCDCs are prepared. Libraries for NGS are prepared using SMART-Seq_V4 Ultra Low Input RNA Kit.

本项目旨在鉴定斑马鱼幼鱼肠道内肠神经元（enteric neurons, ENs）与肠神经嵴来源细胞（enteric neural crest-derived cells, ENCDCs）之间的差异表达基因。我们分别针对两类细胞取材，解剖获取肠神经元对应转基因品系Tg(SAGFF(LF)217B; uas:gfp)以及肠神经嵴来源细胞对应转基因品系Tg(sox10:cre; EF1alpha:loxP-gfp-loxP-dsred)的肠道组织，并分离得到GFP阳性肠神经元与dsRed阳性肠神经嵴来源细胞。两类细胞每组均设置3次生物学重复。采用SMART-Seq_V4 Ultra Low Input RNA Kit构建下一代测序（next-generation sequencing, NGS）文库。