Target mRNAs induce tailing and trimming of Ago2-loaded miRNAs in neurons

We show that target mRNAs trigger the non-templated addition of nucleotides (mainly adenosines and uridines) and shortening of their cognate miRNAs in primary hippocampal neurons. The induced effect is observable both in total RNA and in Ago2-associated RNA, demonstrating that the process is initiated in Ago2 bound miRNAs.我们的研究表明，在原代海马神经元中，靶mRNA可触发其同源微小RNA（miRNAs）发生非模板核苷酸添加（主要为腺苷与尿苷）以及序列截短。该诱导效应在总RNA及Ago2结合RNA中均可观测到，证实这一过程起始于结合了Ago2的微小RNA。