Mapping the human diabetic methylome

Using methyl CpG binding domain capture and deep sequencing (MBD-seq) we examined white blood cells isolated from individuals with or without diabetic complications and non-diabetic controls. Sample where obatined from the Finnish Diabetic Nephropathy Study (FinnDiane) Overall design: Profling differences in genome-wide DNA methylation between diabetics/non-diabetics and diabetics with or without complications

本研究采用甲基化CpG结合域捕获联合深度测序（methyl CpG binding domain capture and deep sequencing，MBD-seq）技术，对从伴或不伴糖尿病并发症患者以及非糖尿病对照个体中分离得到的白细胞进行了检测分析。样本采集自芬兰糖尿病肾病研究（FinnDiane）队列。整体实验设计：解析糖尿病患者、非糖尿病个体，以及伴或不伴并发症的糖尿病患者之间的全基因组DNA甲基化水平差异。