A Genomic Portrait of the Genetic Architecture and Regulatory Impact of microRNA Expression in Response to Infection (functional investigation of the role of miR-29). Homo sapiens

Investigation of the role of miR-29 on transcriptional responses to Mycobacterium tuberculosis (MTB) infection in human dendritic cells using gain- and loss-of-function approaches and microarray expression profiling. Overall design: We assessed the impact of miR-29 perturbation on mRNA expression levels before and after MTB infection in dendritic cells using cells collected from 4 unrelated individuals. Cells were transfected using either a miR-29a mimic or miR-29 family inhibitor before infection with H37Rv for 24 hours. Gene expression was profiled by array and 4 technical replicates were performed to confirm reproducibility.

本研究采用功能获得与功能缺失实验策略，结合微阵列表达谱分析，探究微小RNA-29（miR-29）在人类树突状细胞对结核分枝杆菌（Mycobacterium tuberculosis, MTB）感染的转录应答中的作用。
整体实验设计：我们采集4名无关个体来源的细胞，以此评估miR-29表达扰动对MTB感染前后人类树突状细胞内mRNA表达水平的影响。实验细胞在以H37Rv菌株感染24小时前，分别转染miR-29a模拟物或miR-29家族抑制剂。通过微阵列开展基因表达谱分析，并设置4次技术重复以验证实验结果的可重复性。