TT-seq on COMPASS-degron cells

Here, we use elongation velocity analysis showed that acute loss H3K4me3 caused a general transcriptome-wide attenuation of elongation velocity in cells. In order to examine and monitor the effects of H3K4me3 on newly synthesized RNA rates genome-wide from actively transcribing RNAPII, we performed the modified transient transcriptome sequencing (TTchem-seq) and DRB/TTchem-seq with spike-in strategy on degron cells.

本研究借助延伸速率分析证实，细胞中急性缺失H3K4me3会引发全转录组范围内的转录延伸速率普遍衰减。为了检测并监测H3K4me3对全基因组范围内活跃转录的RNA聚合酶II（RNAPII）所合成的新RNA的生成速率的影响，本研究在降解子细胞（degron cells）中开展了采用spike-in内参策略的修饰型瞬时转录组测序（TTchem-seq）与DRB/TTchem-seq实验。