Modeling of the Nup84 subcomplex of the Nuclear Pore Complex

These scripts demonstrate the use of IMP, MODELLER, and PMI in the modeling of the Nup84 complex using 286 DSS/EDC chemical cross-links and an electron microscopy (EM) 2D class average.

First, MODELLER is used to
generate
initial structures for the individual components in the Nup84 complex.
Then, IMP
is used to model these components using DSS/EDC crosslinks and the
electron microscopy 2D class average for the entire Nup84 complex.

本脚本展示了如何利用IMP、MODELLER与PMI，结合286条DSS/EDC化学交联数据以及电子显微镜（electron microscopy, EM）二维分类平均图，对Nup84复合物开展建模研究。
首先，使用MODELLER生成Nup84复合物各单个组分的初始结构。随后，借助IMP，基于DSS/EDC交联数据与完整Nup84复合物的电子显微镜二维分类平均图，对上述组分进行建模。