Genome-wide maps of ERa-DNA binding events

To identify WT and RNA-binding-domain-mutated (RBDmut) ERa binding sites on DNA, we performed ChIP-seq of FLAG-tagged ERa. Overall design: The endogenous ERa in MCF7 cells were replaced with equal amount of WT and RBDmut FLAG-tagged ERa, and cells were subjected to the ChIP-seq procedure.

为鉴定DNA上野生型（Wild Type, WT）与RNA结合结构域突变型（RNA-binding-domain-mutated, RBDmut）的雌激素受体α（Estrogen Receptor α, ERα）结合位点，我们对携带FLAG标签的ERα实施了染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing, ChIP-seq）。实验设计概述：将MCF7细胞中的内源性ERα替换为等量的野生型及RNA结合结构域突变型FLAG标签ERα，随后将细胞用于染色质免疫共沉淀测序实验。