Chewing Through Challenges: Exploring the Evolutionary Pathways to Wood-Feeding in Insects

This submission includes the partial (V3-V4 regions) 16S rRNA gene libraries of Micromalthus debilis and Epiphragma sp. These sequences were amplified using PCR and subsequently sequenced on the Illumina platform. Our submission includes two biological replicates each for Micromalthus debilis and Epiphragma sp.DNA was extracted using the Quick-DNA Fecal/Soil Microbe Microprep Kit (Zymo research). The V3-V4 regions of the 16S rRNA gene were amplified using PCR primers (S-D-Bact-0341-b-S-17; 5'-CCTACGGGNGGCWGCAG-3' and S-D-Bact-0785-a-A-21; 5'-GACTACHVGGGTATCTAATCC-3; Klindworth et al., 2012). The PCR products were purified and sequenced at Novogene (Beijing, China) using the Illumina MiSeq platform.Taxonomy:Micromalthus debilis: Animalia > Arthropoda > Insecta > Coleoptera > Micromalthidae > Micromalthus > Micromalthus debilisEpiphragma sp.: Animalia > Arthropoda > Insecta > Diptera > Limoniidae > Epiphragma

本提交数据集包含短翅薪甲（Micromalthus debilis）与Epiphragma属物种（Epiphragma sp.）的部分16S核糖体RNA（16S rRNA）基因文库，其扩增区域为V3-V4可变区。所有序列通过聚合酶链式反应（PCR）扩增，并随后在Illumina测序平台完成测序。本数据集为短翅薪甲和Epiphragma属物种各设置了2个生物学重复。基因组DNA采用Quick-DNA粪便/土壤微生物微量制备试剂盒（Zymo Research）提取。16S rRNA基因的V3-V4可变区采用以下PCR引物进行扩增：S-D-Bact-0341-b-S-17（5'-CCTACGGGNGGCWGCAG-3'）与S-D-Bact-0785-a-A-21（5'-GACTACHVGGGTATCTAATCC-3'）（Klindworth等，2012）。PCR扩增产物经纯化后，由位于中国北京的诺禾致源（Novogene）采用Illumina MiSeq测序平台完成测序。分类学信息：
短翅薪甲（Micromalthus debilis）：动物界>节肢动物门>昆虫纲>鞘翅目>短翅薪甲科>薪甲属>短翅薪甲
Epiphragma属物种（Epiphragma sp.）：动物界>节肢动物门>昆虫纲>双翅目>沼大蚊科>Epiphragma属