Design and fabrication of nanostructured surfaces for mammalian and bacterial cellular manipulation

It’s incredibly beneficial addressing single-living cells at a high spatiotemporal resolution while retaining the cell in its native state. Thus, the ultimate goal is to observe cellular responses in contact with nanostructures while using vertically aligned silicon nanowires. However, VA-SiNW are opaque, making non-destructive live-cell characterisation challenging. Additionally, the VA-SiNW can be used as a potential tool to manipulate bacteria cells reducing implant failure caused by biofilm formation. Current treatment strategies include coating surfaces with antibiotics impairing bacterial adhesion temporally. Thus, the research scope of this thesis is to address issues relating to mammalian and bacteria cellular manipulation.