Near-isotropic, reconstructed volume electron microscopy (FIB-SEM) of P7 mouse heart (jrc_mus-heart-1)

This acquisition is part of the CellMap 2024 Segmentation Challenge
Challenge DOI: https://doi.org/10.25378/janelia.c.7456966Challenge Website: https://cellmapchallenge.janelia.org/Sample: Heart tissue from a wild-type, postnatal day 7 mouse, strain: C57BL/6J from Jackson Lab
Sample Description: A dataset featuring isotropic 3D electron microscopy data of the mouse heart (jrc_mus-heart-1). The sample was obtained from a 7-day-old, wild-type C57BL/6J mouse strain sourced from Jackson Laboratory. Mouse anesthesia was performed as IACUC guidelines. Perfusion fixation with glutaraldehyde and vibratome section. After low temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven. The sample was provided by Wei-Ping Li (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia). Post-processing was conducted by Eric Trautman and Stephan Preibisch (HHMI/Janelia).
Protocol: Mouse anesthesia was performed as IACUC guidelines. Perfusion fixation with glutaraldehyde and vibratome section. After low temperature reducing OTO staining, the sample was subject to dehydration, infiltration with graded ethanol and Durcupan resin, and polymerized in a 60°C oven.
Contributions: Sample provided by Wei-Ping Li (HHMI/Janelia), prepared for imaging by Zhiyuan Lu (HHMI/Janelia), and imaged by Wei Qiu (HHMI/Janelia) and post-processing by Eric Trautman and Stephan Preibisch (HHMI/Janelia).
Acquisition ID: jrc_mus-heart-1
Voxel size (nm): 8 x 8 x 8 (x, y, z)
Data dimensions (µm): 163.23 x 151.69 x 135.87 (x, y, z)
Imaging start date: 2023-05-18
Imaging duration (days): 23
Scanning speed (MHz): 3
Dataset URL: s3://janelia-cosem-datasets/jrc_mus-heart-1/jrc_mus-heart-1.zarr/recon-1/em/
Visualization Website: https://openorganelle.janelia.org/datasets/jrc_mus-heart-1
Publication: Xu et al., 2017; Ackerman et al., 2024

本次数据采集隶属于CellMap 2024 分割挑战赛（CellMap 2024 Segmentation Challenge）。
挑战赛DOI：https://doi.org/10.25378/janelia.c.7456966
挑战赛官网：https://cellmapchallenge.janelia.org/
实验样本：取自杰克逊实验室（Jackson Lab）的野生型、出生后7天的C57BL/6J品系小鼠心脏组织。
样本描述：本数据集包含小鼠心脏（jrc_mus-heart-1）的各向同性三维电子显微镜数据。实验样本源自杰克逊实验室的7日龄野生型C57BL/6J品系小鼠。实验按照国际实验动物护理与使用委员会（Institutional Animal Care and Use Committee, IACUC）指南实施小鼠麻醉，采用戊二醛灌注固定并进行振动切片。经低温还原OTO染色后，对样本依次进行梯度乙醇脱水、Durcupan树脂渗透，并置于60℃烘箱中聚合固化。样本由李伟平（Wei-Ping Li，HHMI/Janelia）提供，卢志远（Zhiyuan Lu，HHMI/Janelia）完成成像前样本制备，魏秋（Wei Qiu，HHMI/Janelia）负责成像工作，后续后处理由埃里克·特劳特曼（Eric Trautman）与斯蒂芬·普赖比斯（Stephan Preibisch，HHMI/Janelia）完成。
实验流程：按照IACUC指南实施小鼠麻醉，采用戊二醛灌注固定并进行振动切片。经低温还原OTO染色后，对样本依次进行梯度乙醇脱水、Durcupan树脂渗透，并置于60℃烘箱中聚合固化。
贡献说明：样本由李伟平（HHMI/Janelia）提供，卢志远（HHMI/Janelia）完成成像前样本制备，魏秋（HHMI/Janelia）负责成像工作，后续后处理由埃里克·特劳特曼与斯蒂芬·普赖比斯（HHMI/Janelia）完成。
采集编号：jrc_mus-heart-1
体素尺寸（纳米）：8×8×8（x、y、z轴）
数据维度（微米）：163.23×151.69×135.87（x、y、z轴）
成像起始日期：2023-05-18
成像时长（天）：23
扫描速度（兆赫兹）：3
数据集存储地址：s3://janelia-cosem-datasets/jrc_mus-heart-1/jrc_mus-heart-1.zarr/recon-1/em/
可视化网站：https://openorganelle.janelia.org/datasets/jrc_mus-heart-1
相关文献：Xu等，2017年；Ackerman等，2024年