Dexamethasone does not completely suppress tumor-specific T cell activities in antigen-naïve mice.

Healthy mice received activated pmel-1 T cells labeled with the congenic marker Thy1.1 on day −2, followed by daily administration of dexamethasone (3 mg/kg) from day −2 to day 6. On day 0, mice were vaccinated with gp100, and spleens were harvested for flow cytometry analysis on day 7. Cells were stained with Zombie Yellow for viability, 2NBDG, PE-CD4, PECF594-F4/80, Ly6G, B220, NK1.1, APC-Thy1.1, APC/Cy7-CD8, Pacific Blue-CD3.于第-2天向健康小鼠输注经同源标记Thy1.1（congenic marker Thy1.1）标记的活化pmel-1 T细胞，随后自第-2天至第6天每日给予地塞米松（3 mg/kg）。第0天，小鼠接种gp100疫苗；于第7天收获脾脏并开展流式细胞术分析。实验中使用Zombie Yellow活性染料、2NBDG、PE-CD4、PECF594-F4/80、Ly6G、B220、NK1.1、APC-Thy1.1、APC/Cy7-CD8及Pacific Blue-CD3对细胞进行染色。