Whole genome methylation profiling of melanoma-derived induced pluripotent cancer cells in comparison to the parental melanoma cells

The Illumina Infinium HumanMethylation450 Bead Chip was used to obtain DNA methylation profiles. Samples included melanoma cell lines A375 and HT-144 as well as their reprogrammed progeny and melanoma-iPCC-derived fibroblasts. Pluripotent stem cells and normal human fibroblasts served as references. Genomic DNA from reprogrammed induced pluripotent cancer cells compared to parental melanoma cells, pluripotent stem cells and iPCC-derived fibroblasts as well as normal human fibroblasts.

本研究采用Illumina Infinium 人类甲基化450微珠芯片（Illumina Infinium HumanMethylation450 Bead Chip）获取DNA甲基化谱（DNA methylation profiles）。受试样本包括黑色素瘤细胞系A375、HT-144，二者的重编程子代细胞，以及黑色素瘤诱导多能癌细胞（induced pluripotent cancer cells, iPCC）来源成纤维细胞。多能干细胞（pluripotent stem cells）与正常人类成纤维细胞作为参照样本。本研究对重编程诱导多能癌细胞的基因组DNA，与亲本黑色素瘤细胞、多能干细胞、iPCC来源成纤维细胞及正常人类成纤维细胞开展了对比分析。