Impact of molecular subtypes in muscle-invasive bladder cancer on predicting response and survival outcome to neoadjuvant chemotherapy: results from a multi-institutional validation study. Homo sapiens

Four different molecular classifications of muscle-invasive bladder cancer (MIBC) based on gene expression have been proposed. With the ultimate goal of utilizing these molecular subtypes for personalized treatment, we investigated their significance in the context of neoadjuvant cisplatin-based chemotherapy (NAC). Overall design: RNA was isolated from pre-NAC transurethral resection (TUR) specimens from 305 patients with MIBC and whole transcriptome profiling was performed. Samples were classified according to four published molecular subtyping methods.

目前已有研究基于基因表达特征提出了四种肌层浸润性膀胱癌（muscle-invasive bladder cancer, MIBC）的分子分型方案。为达成将此类分子亚型应用于个性化治疗的最终目标，我们探究了其在基于顺铂的新辅助化疗（neoadjuvant cisplatin-based chemotherapy, NAC）情境中的应用价值。总体实验设计：本研究从305名肌层浸润性膀胱癌患者的新辅助化疗前经尿道切除术（transurethral resection, TUR）标本中提取总RNA，并完成全转录组表达谱分析；随后依据四种已发表的分子分型方法对所有样本进行亚型归类。