Gene expression profiling of E15.5 FoxD1 Cre;Dicer and control embryonic kidneys

The aim of this study is to address the functional role of miRNAs in the FoxD1+ renal stroma progenitors and derivatives during embryonic kidney development. To achieve this, we generated transgenic mice that lack miRNAs in the renal stroma lineage (FoxD1 Cre;Dicer), and performed a microarray analysis on E15.5 whole kidneys to determine the transcriptional changes. 3 litters of E15.5 FoxD1 Cre;Dicer and control littermate kidneys were used for the microarray analysis. Each litter consists of kidneys pooled from 2 embryos per genotype for the RNA extraction.本研究旨在明确微小RNA（miRNAs）在胚胎肾脏发育进程中，于FoxD1阳性肾间质祖细胞及其子代细胞内的功能作用。为实现该研究目的，我们构建了肾间质谱系特异性缺失miRNAs的转基因小鼠（FoxD1 Cre;Dicer），并对胚胎第15.5天（E15.5）的完整肾脏开展基因芯片分析，以鉴定其转录组表达变化。本次基因芯片分析共纳入3窝FoxD1 Cre;Dicer小鼠与同窝对照小鼠的E15.5肾脏样本；每窝样本按基因型分组，每组均混合2个胚胎的肾脏组织以完成RNA提取。