Exploring the impact of biotic and abiotic surfaces on protein binding

The oral environment is composed of a diverse array of proteins, and any substrate inserted into this habitat promptly becomes subjected to protein adsorption and bacterial colonization. However, the predictive and modulatory nature of the surfaces coated with salivary pellicle proteomes in microbial adhesion has not been explored. Thus, using human saliva for salivary pellicle adsorption and for biofilm formation, we compared adsorption and community formation on titanium (Ti) biomaterial (implant devices) and dental surfaces (enamel and dentine). The proteomic profile was evaluated by Liquid chromatography coupled with tandem mass spectrometry and microbiome by 16S RNA sequencing.

口腔环境由多样的蛋白质构成，任何植入该环境的基底材料都会迅速发生蛋白质吸附与细菌定植。然而，目前尚未探究唾液薄膜（salivary pellicle）蛋白质组修饰的表面在微生物黏附过程中的预测与调控特性。因此，本研究以人类唾液开展唾液薄膜吸附与生物膜形成实验，对比了钛（Ti）生物材料（植入器械）与牙体表面（牙釉质及牙本质）上的吸附过程与微生物群落形成情况。本研究通过液相色谱-串联质谱法分析蛋白质组谱，并通过16S RNA测序解析微生物组。