Expression data from vtiligo skin. Expression data from vtiligo skin

Vitiligo is a skin disorder due to loss of melanocytes. There are depigmented lesions mixed with normally-pigmented non-lesions. Gene expression profiles have been different between lesional skin and non-lesional skin. Primary cilia have been involved in various cellular functions including cell survival. We used microarrays to detail the programme of gene expression underlying melanocyte survival in vitiligo and identified distinct cilia-related genes involved in melanocyte apoptosis. Overall design: Lesional and non-lesional skin specimen sets were selected for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain up- or down-regulated gene profiles in lesional skin compared to those in non-lesional skin specimens. To that end, we biopsed lesional and non-lesional skin specimens from the same patient.

白癜风（Vitiligo）是一类因黑素细胞（melanocytes）缺失所导致的皮肤病症。色素脱失的皮损与正常色素沉着的非皮损区域相互混杂。皮损皮肤与非皮损皮肤的基因表达谱存在显著差异。初级纤毛（Primary cilia）参与包括细胞存活在内的多种细胞生物学功能。本研究利用基因芯片（microarrays）详细解析了白癜风中黑素细胞存活相关的基因表达程序，并筛选得到参与黑素细胞凋亡的纤毛相关差异基因。实验设计概述：选取皮损与非皮损皮肤标本进行RNA提取，并在Affymetrix基因芯片上完成杂交检测。本研究旨在获取相较于非皮损皮肤标本，皮损皮肤中上调或下调的基因表达谱。为此，我们从同一患者身上采集了皮损与非皮损皮肤的活检标本。