LET-381/FoxF and its target UNC-30/Pitx2 specify and maintain the molecular identity of C. elegans mesodermal glia that regulate motor behavior

Differential RNAseq analysis to identify transcripts enriched in the GLR glia of C. elegans Overall design: GLR glia were isolated from C. elegans L4 larvae, strain OS11715, by FACS, based on expression of a nuclear YFP marker under control of the GLR glia-specific promoter nep-2prom7. All other cells, which are not expressing YFP, were collected separately for control. RNA reads from YFP-positive and YFP-negative samples were compared to identify GLR glia-enriched genes.

本研究通过差异RNA测序（RNAseq）分析，旨在鉴定秀丽隐杆线虫（C. elegans）GLR胶质细胞中富集的转录本。
实验设计：以GLR胶质细胞特异性启动子nep-2prom7调控的核黄色荧光蛋白（YFP）标记的表达为分选标准，通过荧光激活细胞分选（FACS）从菌株OS11715的秀丽隐杆线虫L4期幼虫中分离GLR胶质细胞；将不表达YFP的其余细胞单独收集作为对照。对比YFP阳性与YFP阴性样本的RNA测序读段，以此鉴定GLR胶质细胞富集的基因。