Hepatic leukemia factor is a novel leukemic stem cell regulator in DNMT3A, NPM1, and FLT3-ITD triple mutated AML

HLF was knocked down in a primary human triple mutated AML sample using shRNA against human HLF versus shRNA against luciferase (shLuc) as control to determine gene expression changes 72 hours after lentiviral transduction. Overall design: Transcriptome profiling of shRNA mediated HLF knockdown cells in primary AML cells.

本研究针对携带三重突变的原代人急性髓系白血病（Acute Myeloid Leukemia, AML）样本，采用靶向人HLF的短发夹RNA（short hairpin RNA, shRNA）进行HLF基因敲低，以靶向荧光素酶的短发夹RNA（shLuc）作为对照，旨在探究慢病毒转导72小时后的基因表达变化情况。整体实验设计：对经短发夹RNA介导HLF基因敲低的原代AML细胞开展转录组谱分析。