Transcriptional profile Lactobacillus plantarum WCFS1 : Hydroxytyrosol treated cells. Lactiplantibacillus plantarum

Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with hydroxytyrosol. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with hydroxytyrosol. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. The transcriptional profile reveals the induction of genes related to defense mechanisms against oxidative challenge. This response include genes related to inactivation of toxic oxygen radicals, probably derived from the autooxidation of hydroxytyrosol and genes involved in repairing the damage of oxygen radicals on proteins, including those involved in the metabolism of sulphur amino acids. Genes involved in peptidoglycan turnover, strenghthening of the cell wall and genes coding for osmoprotectants transporters, were downregulated. In addition, genes coding for elements typically involved in the stringent response, including ribosomal proteins, enzymes involved in the metabolism of the ppGpp alarmone, nitrogen metabolism, cell division and general stress proteins, were differentially regulated by hydroxytyrosol. Overall design: Biological triplicates: untreated cells: Control vs. Hydroxytyrosol treated cells: Experimental

采用全基因组转录谱分析（Whole genome transcriptional profiling）对人类分离株植物乳杆菌WCFS1（Lactobacillus plantarum WCFS1）在羟基酪醇（hydroxytyrosol）胁迫下的应答特征进行解析。本研究共开展12次独立实验，随后按每4组随机混合，最终得到3份RNA样本。采用全基因组转录谱分析对人类分离株植物乳杆菌WCFS1在羟基酪醇胁迫下的应答特征进行解析。本研究共开展12次独立实验，随后按每4组随机混合，最终得到3份RNA样本。该转录谱显示，与氧化胁迫防御相关的基因被诱导表达。该应答包含与毒性氧自由基灭活相关的基因（这类自由基大概率源于羟基酪醇的自氧化），以及参与修复氧自由基对蛋白质造成损伤的基因，其中包括参与硫氨基酸代谢的基因。参与肽聚糖（peptidoglycan）周转、细胞壁加固的基因，以及编码渗透压保护物质转运蛋白的基因均被下调表达。此外，通常参与严紧反应（stringent response）的编码基因，包括核糖体蛋白、ppGpp警报素（ppGpp alarmone）代谢相关酶、氮代谢相关酶、细胞分裂相关蛋白及通用应激蛋白，均受到羟基酪醇的差异化调控。整体实验设计：生物学重复三组，未处理细胞作为对照组，羟基酪醇处理细胞作为实验组。