Down-regulation of SFRP1 in a mammary epithelial cell line. Homo sapiens

Microarray data to accompany Gauger et al. (2009) Cancer Cell International paper Overall design: A treated X untreated design was used whereby immortalized human mammary epithelial cell lines (TERT cells) were modified to stably express an siRNA that knocked down SFRP1 expression. These "treated" cells were labeled with Cy5 and four replicate harvests were hybridized to Agilent 4by44k whole genome microarrays, along with a Cy3 labeled untreated control. The untreated control consisted of 4 pooled harvests of the parent cell line in normal growth conditions at 60-80% confluence.

本数据集为配套Gauger等人2009年发表于《Cancer Cell International》的研究论文的微阵列数据。实验设计概况：本研究采用处理组-对照组实验设计，将永生化人乳腺上皮细胞系（TERT细胞）进行基因修饰，使其稳定表达可敲低SFRP1基因表达的小干扰RNA（small interfering RNA，siRNA）。将上述“处理组”细胞以Cy5荧光标记，将四次重复收获的处理组样品与Cy3标记的对照组样品，共同在安捷伦（Agilent）4×44k全基因组微阵列芯片上完成杂交。对照组为亲本细胞系在正常培养条件下（细胞汇合度60%~80%）收集的四次混合样品。