Clinical significance and biological function of transcriptional repressor GATA binding 1 in gastric cancer: a study based on data mining, RT-qPCR, immunochemistry, and vitro experiment

Transcriptional repressor GATA binding 1 (TRPS1) is a newly discovered transcription factor, which has been reported in many tumors, except for gastric cancer (GC). In this study, we aimed to grope for clinical significance and biological function of TRPS1 in GC. TRPS1 expression in GC and its relationship with clinicopathological features were analyzed based on public databases, and verified by immunohistochemistry and RT-qPCR. Kaplan-Meier survival curve and Cox regression model were used to estimate the influence of TRPS1 on the univariate prognosis and multivariate survival risk factors of GC. The effects of TRPS1 on malignant biological behaviors of GC cells were studied by CCK8 cell proliferation, scratch test, and Transwell assay. The function of TRPS1 was further analyzed by signaling pathway analysis. TRPS1 mRNA expression in GC tissues was up-regulated and was of great significance in some prognostic factors. Protein expression of TRPS1 in tumor tissues was significantly higher than that in paracancerous tissues. Over-expression of TRPS1 was a poor prognostic indicator for GC patients. TRPS1 knockdown could inhibit the proliferation, migration, and invasion of GC cells. The important role of TRPS1 was in the extracellular matrix, and it was involved in actin binding and proteoglycan in cancer. The hub genes of TRPS1 (FN1, ITGB1) were defined. TRPS1 may be a tumor promoter and promote the development of GC by influencing the malignant biological behaviors of GC. TRPS1 is expected to be a key diagnostic and prognostic indicator for GC patients.

GATA结合蛋白1转录抑制因子（TRPS1）是一种新发现的转录因子，已在多种肿瘤中被报道，但目前尚未见其在胃癌（GC）中的相关研究。本研究旨在探索TRPS1在胃癌中的临床意义与生物学功能。研究基于公共数据库分析了胃癌组织中TRPS1的表达水平及其与临床病理特征的关联，并通过免疫组化（immunohistochemistry）与实时定量聚合酶链反应（RT-qPCR）进行验证。采用Kaplan-Meier生存曲线与Cox回归模型，评估TRPS1对胃癌患者单因素预后及多因素生存风险的影响。通过CCK8细胞增殖实验、划痕实验及Transwell实验探究TRPS1对胃癌细胞恶性生物学行为的影响，并进一步通过信号通路分析解析TRPS1的作用机制。结果显示，胃癌组织中TRPS1的mRNA表达水平显著上调，且与多项预后因素密切相关；肿瘤组织中TRPS1的蛋白表达水平显著高于癌旁组织。TRPS1高表达是胃癌患者预后不良的预测指标。敲低TRPS1可抑制胃癌细胞的增殖、迁移与侵袭能力。TRPS1主要通过细胞外基质发挥作用，且在肿瘤进程中参与肌动蛋白结合与蛋白聚糖相关调控。本研究明确了TRPS1的枢纽基因（FN1、ITGB1）。TRPS1可作为胃癌促癌因子，通过调控胃癌细胞的恶性生物学行为促进肿瘤进展，有望成为胃癌患者重要的诊断与预后标志物。