RNA sequencing profile of cord blood endothelial progenitor cells. RNA sequencing profile of cord blood endothelial progenitor cells

Endothelial colony forming cells (ECFC) are circulating endothelial progenitors that are recruited to sustain endothelial function, vascular growth and repair. They are particularly abundant in the perinatal period and can be isolated from umbilical cord blood, thus representing neonatal ECFC. In this study, we profiled neonatal ECFC depending on maternal metabolic derangements to investigate intrauterine epigenetic programming. Overall design: ECFC were isolated from umbilcal cord blood after pregnancies of lean, overweight and GDM (gestational diabetes mellitus) developing mothers with male and female neonates each.

内皮集落形成细胞（Endothelial colony forming cells, ECFC）是一类循环内皮祖细胞，可被招募以维持内皮功能、血管生成及组织修复。该细胞群在围产期含量尤为丰富，可从脐带血中分离获取，因此又被称为新生儿ECFC。本研究针对不同母体代谢紊乱状态下的新生儿ECFC开展表征分析，以探究宫内表观遗传编程的相关机制。实验设计概述：本研究从妊娠期间体质量正常、超重及罹患妊娠糖尿病（gestational diabetes mellitus, GDM）的产妇所分娩的男、女新生儿脐带血中分离ECFC，各分组均纳入男、女新生儿。