Metagenome assembled genomes co_3-Bin_17_1.fa

Co-assemblies were binned with CONCOCT within anvi’o v5.1 by clustering scaffolds 2500 bp or longer into metagenome assembled genomes (MAGs) and manually refining them within the anvi’o platform. Furthermore, dRep v2.3.2 was used to dereplicate MAGs and obtain representative genomes with flags ‘-comp 50 -con 10’ and default values.

共组装序列（Co-assemblies）在anvi’o v5.1环境中借助CONCOCT工具完成分箱操作：将长度不低于2500 bp的序列支架（scaffolds）聚类为宏基因组组装基因组（metagenome assembled genomes, MAGs），并在anvi’o平台内对其进行手动精修。此外，使用dRep v2.3.2工具对上述宏基因组组装基因组（MAGs）进行去冗余处理，通过指定参数‘-comp 50 -con 10’并保留默认参数，以获取代表性基因组。