CD73-Dependent Adenosine Signaling through Adora2b Drives Immunosuppression in Ductal Pancreatic Cancer

In this experiment, we evaluted whole transcriptomic changes in ex vivo cultured ducts expressing mutant Kras compared to control ducts. The cells were cultured for 7 days before RNA was isolated for RNA-seq. For the ex vivo cultured ducts, Hnf1b:CreERT2; LSL-KrasG12V mice were given 10 mg tamoxifen or corn oil control and euthanized at day 4 for ex vivo culturing of ducts.

本实验中，我们对比了表达突变型KRAS的离体（ex vivo）培养导管与对照导管的全转录组变化。实验前将导管相关细胞培养7天，随后提取RNA进行RNA测序（RNA-seq）。针对用于离体培养的导管，我们向Hnf1b:CreERT2; LSL-KrasG12V 小鼠注射10 mg他莫昔芬或玉米油作为对照，并于给药后第4天对小鼠实施安乐处死，以获取导管用于后续离体培养。