Details of the primer sets used for reverse transcription loop-mediated isothermal amplification (RT-LAMP) including the primers tagged for nucleic acid lateral flow (NALF) detection.

The HIV and β-actin primer sets are modified for lateral flow detection by the addition of biotin, fluorescein isothiocyanate (FITC), and digoxigenin (DIG).
Details of the primer sets used for reverse transcription loop-mediated isothermal amplification (RT-LAMP) including the primers tagged for nucleic acid lateral flow (NALF) detection.

针对人类免疫缺陷病毒（HIV）与β肌动蛋白（β-actin）的引物组，通过添加生物素、异硫氰酸荧光素（FITC）以及地高辛配基（DIG）进行修饰，以适配侧流检测。本文详述了用于逆转录环介导等温扩增（RT-LAMP）的引物组相关细节，其中涵盖了用于核酸侧流（NALF）检测的标记引物。