RNAseq of mouse colon epithelial cell fraction in Lingo3, and WT mice. RNAseq of mouse colon epithelial cell fraction in Lingo3, and WT mice

The purpose of this experiment is to deep sequence transcripts in WT mice and mice lacking Lingo3, and compare gene expression in the epithelial cells of the colon between wild type and mutant mice. Colon tissue was isolated from each mouse, and epithelial cells were removed from the colon by washing the tissue with 5 mM EDTA + 2.5 mM DTT solution. Overall design: wild type and Lingo3 mutant mice, 3 replicates for each condition, 6 samples total

本实验旨在对野生型（WT）及Lingo3基因缺失小鼠的转录本进行深度测序，进而比较野生型与突变型小鼠结肠上皮细胞的基因表达差异。从每只小鼠体内分离结肠组织后，通过以5 mM乙二胺四乙酸（EDTA）+2.5 mM二硫苏糖醇（DTT）溶液冲洗组织的方式，从结肠中分离得到上皮细胞。本实验的整体设计为：设置野生型与Lingo3突变型小鼠两组，每组设置3次生物学重复，总计6个样本。