AML5_DMSO vs UM171. AML5_DMSO vs UM171

UM171 effect on AML5 cell lines (kinetic) Overall design: OCI-AML5 cell line and OCI-AML5 cells engineered to express sgRNA against EPCR (EPCR null cell) were exposed to DMSO or UM171. RNA were extracted from sorted subsets and sequences were obtained with an illumina HiSeq 2000 sequencer.UM171对AML5细胞系的影响（动力学研究） 实验设计概述：将OCI-AML5野生型细胞系以及经基因工程改造以表达靶向内皮细胞蛋白C受体（endothelial protein C receptor，EPCR）的单导向RNA（single guide RNA，sgRNA）的OCI-AML5细胞（即EPCR敲除细胞）分别经二甲基亚砜（dimethyl sulfoxide，DMSO）或UM171处理。从分选得到的细胞亚群中提取RNA，并通过Illumina HiSeq 2000测序仪完成测序。