Demographic Factors Affect the BMAC Proteome Dataset

CSV file is the result of each text file.

Wet work: BMAC samples were spun to searate out cellular and serum component. The serum was then filtered through an IgG and albumin exclusion column before performing a bicinchoninic acid assay to determine each samples concentration. All samples were diluted to a uniform concentration and applied to one of four antibody-microarrays.

Data processing: Microarrays were scanned in GenePix4000b scanner using the GenePix Pro 6.0.1.25 software. Raw data was exported and the 'F52 Mean- - B532' data was used. Proteins were analyzed in quadruplicate, all spots greater than 20% of the median value of their sister spots were excluded. Spots which met the inclusion criteria were averaged and a log2 transform was applied before normalizing all spots to the average of two control samples run on each array.

*Note features in excel file have been obfuscated, all values remain, but names have be anonymized for future publication purposes*

本数据集包含的CSV文件均源自各文本文件的处理结果。

湿实验流程：BMAC样本经离心分离得到细胞组分与血清组分。将血清通过IgG与白蛋白去除色谱柱过滤后，采用双喹啉甲酸（bicinchoninic acid, BCA）蛋白定量实验测定各样本的蛋白浓度。将所有样本稀释至统一浓度，随后点样至四块抗体微阵列（antibody-microarrays）中的一块进行实验。

数据处理流程：使用GenePix Pro 6.0.1.25软件，在GenePix4000b扫描仪中完成微阵列扫描。导出原始数据后，选取'F52 Mean-B532'数据开展后续分析。蛋白检测设置四重重复，所有信号强度高于对应姐妹斑点中位数20%的斑点均予以排除。对符合纳入标准的斑点信号取平均值，随后进行log₂对数转换，最后将所有斑点的信号值标准化至每块微阵列上两个对照样本的平均信号水平。

*备注：Excel文件中的特征名称已做混淆处理，所有数值均完整保留，但为便于后续发表，已将相关名称进行匿名化处理。