affy_riz_2011_7-: Transcriptomic analysis of rice leaves challenged with virulent and avirulent Xoo strains. Oryza sativa

affy_riz_2011_7 - affy_riz_2011_7 - The Bacterial Leaf Blight disease of rice is due to Xanthomonas oryzae pv. oryzae. As for many pathogenic bacteria, it relies on a type 3 secretion system (TTSS) that is devoted to the injection of type 3 effectors (T3Es) into the eukaryotic host cell. These proteins are meant to suppress host basal defense responses and/or mimic some host regulatory function promoting bacterial survey in the plant. During an incompatible interaction, T3Es may act as Avr proteins and stimulate Effector-Triggered-Immunity. We aim at evaluating the transcriptomic response of rice leaves challenged with avirulent strains of Xoo BAI3 and MAI1 on resistant lines IR64 and IRBB4 versus the reference susceptible rice line Nipponbare. In addition, we investigated the transcriptomic response of rice leaves upon inoculation of an XoohrcC mutant strain affected in the production of a functional TTSS.-The goal of the experiment is to characterize the rice leaf transcriptome response, upon the inoculation of susceptible and resistant rice leaves 24 hours post-infection. To that end, the experimental design includes the inoculation of susceptible Nipponbare rice leaves with Xoo strains BAI3 (race A1) and MAI1 (race A3), that will be compared to the response of resistant lines IRBB4 and IR64 rice lines. In addition, Nipponbare rice leaves will also be challenged with the BAI3hrcC mutant that is affected in the production of a functional TTSS. Overall design: 18 arrays - rice; avirulent vs virulent

affy_riz_2011_7 - 本数据集围绕水稻白叶枯病展开研究，该病的致病菌为水稻黄单胞杆菌水稻致病变种（Xanthomonas oryzae pv. oryzae，简称Xoo）。与多数病原细菌一致，该致病菌依赖III型分泌系统（Type 3 Secretion System, TTSS），将III型效应蛋白（Type 3 Effectors, T3Es）注入真核宿主细胞。此类效应蛋白的功能为抑制宿主基础防御反应，或模拟宿主调控功能以促进细菌在植物体内的定殖。在非亲和互作过程中，III型效应蛋白可作为无毒蛋白（Avr蛋白）触发效应子触发的免疫反应（Effector-Triggered Immunity, ETI）。本研究旨在评估：在抗病水稻品系IR64、IRBB4与易感对照品种日本晴（Nipponbare）的叶片上，接种Xoo无毒菌株BAI3与MAI1后，水稻叶片的转录组响应。此外，本研究还探究了接种功能III型分泌系统缺陷的XooΔhrcC突变菌株后，水稻叶片的转录组变化。
本实验的核心目标为：解析感病与抗病水稻叶片在接种病原菌24小时后的转录组响应。实验设计如下：将易感品种日本晴的叶片分别接种Xoo菌株BAI3（小种A1）与MAI1（小种A3），并将其转录组响应与抗病品系IRBB4、IR64的叶片响应进行对比；同时，将日本晴叶片接种BAI3ΔhrcC突变菌株（该菌株无法产生功能正常的III型分泌系统）。实验整体设计：共18张基因芯片，样本来源为水稻，比较组涵盖无毒菌株与致病菌株处理组。