Whole transcriptome analysis of neuroblastoma cell lines after ERK5 silencing. Whole transcriptome analysis of neuroblastoma cell lines after ERK5 silencing

The transcriptomic network modulated after the depletion of the tyrosin kinase ERK5 through shRNA-mediated silencing was analyzed in SK-N-BE(2) and CHLA-90 neuroblastoma cell lines, in order to characterize and reveal any relevant role of this kinase at the transcriptional level. Overall design: SK-N-BE(2) and CHLA-90 cells were transduced with lentiviral particles carrying two different shRNA for ERK5 silencing and 72 h later RNA was extracted in biological triplicates for expression microarray analysis.

本研究针对SK-N-BE(2)与CHLA-90两种神经母细胞瘤细胞系，分析了通过短发夹RNA（shRNA）介导的沉默技术敲低酪氨酸激酶ERK5后所调控的转录组网络，旨在表征并揭示该激酶在转录层面的关键作用。实验设计概况：将携带两种不同靶向ERK5沉默用短发夹RNA的慢病毒颗粒转导至SK-N-BE(2)与CHLA-90细胞中，72小时后提取RNA并设置三份生物学重复，用于基因表达微阵列分析。