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Additional file 2 of Mechanism of differential expression of β-glucosidase genes in functional microbial communities in response to carbon catabolite repression

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Additional file 2: Table S1. Primers of β-glucosidase genes for qPCR. Table S2. The ratio of glucose-tolerant β-glucosidase genes of different treatments at T1, T2, and C phase from DNA library. Table S3. Results and statistics of qPCR and qRT-PCR of treatment groups in T1 phase. Table S4. Results and statistics of qPCR and qRT-PCR of treatment groups in T2 phase. Table S5. Results and statistics of qPCR and qRT-PCR of treatment groups in C phase. Table S6. Identity of 28 GH1 family β-glucosidase genes with a characteristic sequence that can identify glucose tolerant or non-glucose tolerant detected in both metagenome and metatranscriptome database. Table S7. GH1 family β-glucosidase genes with a characteristic sequence that can identify glucose tolerant or non-glucose tolerant detected in the metatranscriptome database. Table S8. GH1 family β-glucosidase genes with a characteristic sequence that can identify glucose tolerant or non-glucose tolerant detected in the metagenome database.

附加文件2:表S1。用于定量聚合酶链反应(quantitative PCR,qPCR)的β-葡萄糖苷酶基因引物。表S2:不同处理组在T1、T2及C时期,基于DNA文库得到的耐葡萄糖β-葡萄糖苷酶基因占比。表S3:T1时期各处理组定量聚合酶链反应(quantitative PCR,qPCR)与实时定量反转录聚合酶链反应(quantitative reverse transcription PCR,qRT-PCR)的结果及统计数据。表S4:T2时期各处理组定量聚合酶链反应(quantitative PCR,qPCR)与实时定量反转录聚合酶链反应(quantitative reverse transcription PCR,qRT-PCR)的结果及统计数据。表S5:C时期各处理组定量聚合酶链反应(quantitative PCR,qPCR)与实时定量反转录聚合酶链反应(quantitative reverse transcription PCR,qRT-PCR)的结果及统计数据。表S6:在宏基因组与宏转录组数据库中均检出的、携带有可区分耐葡萄糖与非耐葡萄糖特征序列的28个GH1家族β-葡萄糖苷酶基因的序列一致性信息。表S7:在宏转录组数据库中检出的、携带有可区分耐葡萄糖与非耐葡萄糖特征序列的GH1家族β-葡萄糖苷酶基因。表S8:在宏基因组数据库中检出的、携带有可区分耐葡萄糖与非耐葡萄糖特征序列的GH1家族β-葡萄糖苷酶基因。
提供机构:
Li, Shanshan; Bello, Ayodeji; Liu, Jiawen; Ma, Bo; Gao, Liyuan; Liu, Libo; Li, Hongtao; Chen, Xiehui; Zhang, Xinyue; Fan, Zhihua; Wang, Shouzhi
创建时间:
2022-01-13
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