RNA sequencing analysis of unilateral orchidectomy, bilateral orchidectomy, and Nell2 knockout initial segment-caput epididymis.. RNA sequencing analysis of unilateral orchidectomy, bilateral orchidectomy, and Nell2 knockout initial segment-caput epididymis.

Purpose: The purpose of this study is to characterize genes whose expressions in the initial segment (IS)-caput epididymis were affected by unilateral orchidectomy (UOD), bilateral orchidectomy (BOD), and Nell2 gene ablation. Methods: The unilateral or bilateral orchidectomy of WT mice was performed from 8 weeks old and continued for 4 weeks until tissue sampling at 12 weeks old. Contralateral epididymis was used as a control for UOD. Sham operation was also done as a control for BOD. Nell2-/- mice and their control WT mice were used wthen they were 14-week-old. IS-caput epididymal mRNA profiles were generated by deep sequencing, in triplicate, using Illumina NovaSeq6000. Results: RNA-seq data identified differentially expressed transcripts. Conclusions: Our results show that the expression of many genes was affected by UOD, BOD, and Nell2 ablation. Overall design: IS-caput epididymal mRNA profiles of mice with experimental treatments were generated by deep sequencing, in triplicate, using Illumina NovaSeq6000.

研究目的：本研究旨在明确受单侧睾丸切除术（unilateral orchidectomy, UOD）、双侧睾丸切除术（bilateral orchidectomy, BOD）及Nell2基因敲除（Nell2 gene ablation）影响的小鼠附睾头起始段（initial segment-caput epididymis）基因表达特征。
实验方法：对8周龄野生型（wild type, WT）小鼠实施单侧或双侧睾丸切除术，术后饲养4周，于12周龄时采集组织样本。以对侧附睾作为单侧睾丸切除术的对照，同时设置假手术组作为双侧睾丸切除术的对照。实验使用14周龄的Nell2基因敲除（Nell2-/-）小鼠及其同窝野生型对照小鼠。采用Illumina NovaSeq6000平台对附睾头起始段的mRNA进行深度测序，每组设置3次生物学重复。
实验结果：通过RNA测序（RNA-seq）数据鉴定出差异表达转录本。
研究结论：本研究结果表明，单侧睾丸切除术、双侧睾丸切除术及Nell2基因敲除均可影响大量基因的表达水平。
整体实验设计：采用Illumina NovaSeq6000平台对经不同实验处理的小鼠附睾头起始段mRNA进行深度测序，每组设置3次生物学重复。