RNA degradosome components purified from Mycobacterium tuberculosis

The dataset accompanying a publication describing the RNA degradosome components in Mycobacterium tuberculosis (Mtb). Individual RNA degradosome components from Mtb (PNPase, RNase E, RNase J, RhlE and Enolase) were tagged with eGFP and used as baits to pull-down their interaction partners. The protein-complexes were purified by affinity chromatography, using previously published protocols, and submitted to LC-MS analysis.

本数据集配套于一篇阐述结核分枝杆菌（Mycobacterium tuberculosis, Mtb）内RNA降解体（RNA degradosome）组分的研究论文。研究人员将结核分枝杆菌的各RNA降解体组分——涵盖PNPase、RNase E、RNase J、RhlE与烯醇化酶（Enolase）——均标记eGFP标签，并作为诱饵蛋白通过下拉实验（pull-down）富集其互作伴侣蛋白。随后遵循已发表的实验方案，通过亲和层析（affinity chromatography）纯化得到蛋白质复合物，并将其提交至LC-MS（液相色谱-质谱联用）分析。