Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ada2b]. Chiffon triggers global histone H3 acetylation and expression of developmental genes in Drosophila embryos [ada2b]

Purpose: The goal of this study was to identify the differential expressed genes in the Drosophila embryo when ada2b is disrupted. Overall design: Method: RNA extracted from Drosophila embryos (stage 12-14) was used to generate cDNA libraries using NuGen Ovation RNA-seq System V2. Samples were sequenced using Ilumina HiSeq150 next generation sequencer (four biological replicates per genotype: ada2b null mutants flies, and the rescue of different transgenes: Ada2b_PA, Ada2b_PB, A34908116da2b_PA+Ada2b_PB, and a WT control.

研究目的：本研究旨在鉴定ada2b基因功能缺失后的果蝇胚胎中的差异表达基因（differentially expressed genes）。
实验设计：提取果蝇12-14期胚胎的RNA，利用NuGen Ovation RNA-seq System V2试剂盒构建cDNA文库。采用Illumina HiSeq150新一代测序仪进行测序，每组基因型设置4个生物学重复：ada2b纯合无效突变体果蝇、不同转基因回补株系（Ada2b_PA、Ada2b_PB、A34908116da2b_PA+Ada2b_PB）以及野生型（WT）对照。