Micness samples: 47 samples of drosophila melanogaster & cervus elaphus raw sequence reads

To evaluate the performance of the Micness method, we compared the genotypes assignedby MicNeSs to the genotypes obtained from the GCS method. We amplified several target microsatellite loci by PCR and sequenced them in parallel by capillary sequencing and by 454 pyrosequencing, which produces longer reads than other NGS methods. Seven loci were ampli fied from inbreed lines of Drosophila melanogaster, and six from a natural species, Cervus elaphus.

为评估Micness方法（Micness method）的性能，我们将MicNeSs所分配的基因型与GCS方法（GCS method）得到的基因型进行了比对分析。我们通过聚合酶链式反应（Polymerase Chain Reaction, PCR）扩增了多个靶标微卫星位点，并采用毛细管测序与454焦磷酸测序两种手段进行平行测序——相较于其他下一代测序（Next-Generation Sequencing, NGS）方法，454焦磷酸测序可产生更长的测序读段。我们从黑腹果蝇（Drosophila melanogaster）的近交系中扩增得到7个位点，从天然物种马鹿（Cervus elaphus）中扩增得到6个位点。