Analysis of differential expressed genes between ad- and abaxial rosette leaves

To analysis of differential expressed genes between ad- and abaxial rosette leaves, we sequenced the adaxial side and abaxial side of leaves from twenty 4-week-old A. thaliana [L.] Heynh. ecotype Columbia plants. With the use of double-sided tape and a blade, the tissue on the abaxial and adaxial sides was collected separately. Total RNA was extracted from the two sides of leaves using the TransZol reagent.

为分析莲座叶近轴面（adaxial）与远轴面（abaxial）之间的差异表达基因，我们对20株4周龄的拟南芥（Arabidopsis thaliana [L.] Heynh.）哥伦比亚生态型植株的叶片近轴面与远轴面进行了测序。采用双面胶带与解剖刀片分别收集叶片远轴面与近轴面的组织，使用TransZol试剂从叶片的两个面中提取总RNA。