Lettuce 16S rRNA gene amplicon sequence reads Raw sequence reads

This study aims to determine the effect of post-harvest washing and irradiation on the bacterial community, with a focus on the potential of these interventions to mitigate ARB and ARGs on leafy vegetables. To simulate real-world sources of contamination such as contact with compost either in soil or within a post-harvest wash situation, lettuce leaves were inoculated with compost slurry containing antibiotic-resistant strains of pathogenic and spoilage microorganisms of concern, E. coli O157:H7 and Pseudomonas aeruginosa. Changes to the relative abundance of taxonomic groups on the lettuce phyllosphere of irradiated and non-irradiated lettuce stored under modified atmosphere conditions at 4ºC was assessed by 16S rRNA gene amplicon sequencing.

本研究旨在探究收获后清洗与辐射处理对细菌群落的影响，并着重评估这些干预措施在减轻叶菜类蔬菜上抗生素耐药菌（ARB）和抗生素耐药基因（ARGs）的潜在作用。为模拟现实世界中的污染来源，如与土壤中或收获后清洗过程中堆肥的接触，本研究将生菜叶片接种了含有致病性及腐败微生物、抗生素耐药菌株的堆肥悬浮液，包括大肠杆菌O157:H7和铜绿假单胞菌。通过16S rRNA基因扩增子测序技术，评估了在4℃的改良大气条件下储存的辐照与非辐照生菜叶片表面（叶表面群落）中分类群相对丰度的变化。