Transcriptome Sequencing of Optic Nerve in a Rat Model of Neuromyelitis Optica Spectrum Disorder-Related Optic Neuritis (NMOSD-ON)

This study established a rat model of NMOSD-ON by injecting patient-derived AQP4+ serum into the optic nerve sheath of rats. Optic nerve tissues were collected at 24 hours and 7 days post-modeling, along with a blank control group, for transcriptome sequencing. The aim was to explore gene expression changes during the early and peak phases of NMOSD-ON pathogenesis, thereby identifying potential disease mechanisms. Experimental design: The study included three groups: (1) healthy control group, (2) NMOSD-ON model group at 24 hours post-induction, and (3) NMOSD-ON model group at 7 days post-induction. Each group consisted of three biological replicates, totaling nine samples. The NMOSD-ON model was induced by injection of AQP4+ serum into the optic nerve sheath. Optic nerve tissues were collected for RNA-Seq analysis to compare gene expression profiles between the groups.

本研究通过向大鼠视神经鞘内注射患者来源的水通道蛋白4（Aquaporin 4，AQP4）阳性血清，构建了视神经脊髓炎谱系疾病相关性视神经炎（NMOSD-ON）大鼠模型。于造模后24小时及7天采集视神经组织，同时设置空白对照组，开展转录组测序实验。本研究旨在探究NMOSD-ON发病早期与峰值阶段的基因表达变化，进而阐明潜在的疾病致病机制。
实验设计：本研究共设置三组实验分组：(1) 健康对照组；(2) 造模后24小时的NMOSD-ON模型组；(3) 造模后7天的NMOSD-ON模型组。每组包含3个生物学重复，总计9个样本。本研究通过向大鼠视神经鞘内注射AQP4+血清构建NMOSD-ON模型，采集视神经组织进行RNA测序（RNA-Seq）分析，以比较各组间的基因表达谱差异。