RNA sequencing of AARS1 knockdown in ovarian cancer cell lines

Although alanyl-transfer RNA synthetase (AARS)1/2 are known to act as cellular lactate sensors, their role in high-grade serous ovarian cancer (HGSOC) remains unclear.Therefore, this study explored the role of AARS1 in HGSOC-associated protein lactylation and examined its effects on the growth and progression of HGSOC. We used high-throughput sequencing to identify ATF5 as the major downstream target gene regulated by AARS1 in ovarian cancer.

尽管丙氨酰转移RNA合成酶（alanyl-transfer RNA synthetase，AARS）1/2已被证实可作为细胞乳酸感受器，但其在高级别浆液性卵巢癌（high-grade serous ovarian cancer，HGSOC）中的作用仍未明确。因此，本研究探讨了AARS1在高级别浆液性卵巢癌相关蛋白质乳酸化（protein lactylation）中的作用，并考察了其对高级别浆液性卵巢癌生长与进展的影响。我们通过高通量测序（high-throughput sequencing）鉴定出激活转录因子5（activating transcription factor 5，ATF5）是卵巢癌中受AARS1调控的主要下游靶基因。