Transcriptome profiling reveal the effect of Sutent in the mice adipose tissues and livers

We administered clinically relevant dose of sutent (a small molecule inhibitor of multi-target receptor tyrosine kinases) to mice daliy for 16 weeks. We then compared the effect of sutent on four tissues(BAT,iWAT,eWAT and liver) transcriptome using RNAseq. We found sutent treatment enhances energy expenditure and improves metabolism in mice under HFD. Results provide insight into the molecular mechanisms of sutent in the treatment of obesity. Overall design: Four tissues from 8 mice:4 mice were treated with sutent, 4 mice were treated with vehicle.我们向小鼠每日给予临床相关剂量的舒尼替尼（Sutent，一种多靶点受体酪氨酸激酶小分子抑制剂），连续给药16周。随后通过RNA测序（RNA-seq）比较舒尼替尼对四种组织——棕色脂肪组织（BAT，brown adipose tissue）、腹股沟白色脂肪组织（iWAT，inguinal white adipose tissue）、附睾白色脂肪组织（eWAT，epididymal white adipose tissue）及肝脏——转录组的影响。研究发现，在高脂饮食（HFD，high-fat diet）喂养的小鼠中，舒尼替尼处理可增强能量消耗并改善代谢水平。本研究结果为阐明舒尼替尼治疗肥胖的分子机制提供了新的见解。整体实验设计：采集8只小鼠的四种组织样本，其中4只接受舒尼替尼处理，另外4只给予溶剂对照处理。