Gene expression profiling from pooled samples of liver tissue of liver MyD88 WT mice and MyD88 liver specific KO mice fed either with a control diet or a high-fat diet.. Mus musculus

Mice wild type or knocked-out for the MyD88 gene specifically in liver, were recruited for this expression profiling experiment. Each group of mice (WT versus LKO) were fed with a control diet or a high fat diet. Then mice were sacrificed and liver samples form were processed for RNA extraction. Total liver RNA of each sample was then pooled with those of the same group and treatment for microarray hybridization. Overall design: four groups of mice: (I) WT-CT, (II) MyD88 liver specific KO (LKO-CT), (III) WT treated with high-fat diet (WT-HFD) and (IV) MyD88 liver specific KO treated with high-fat diet (LKO-HFD) .

本表达谱实验招募了两类小鼠：野生型（wild type, WT）小鼠，以及肝脏特异性敲除髓系分化因子88（MyD88）基因的小鼠。将两组小鼠（WT与肝脏特异性敲除型（liver-specific knocked-out, LKO））分别饲喂对照饲料与高脂饲料（high fat diet, HFD）。随后处死小鼠，采集肝脏样本并进行RNA提取。将同组且接受相同处理的所有样本的总肝脏RNA混合后，用于芯片杂交（microarray hybridization）。实验整体设计分为四组小鼠：(I) 野生型对照（WT-CT）、(II) 肝脏特异性MyD88敲除对照（LKO-CT）、(III) 高脂饲料处理的野生型小鼠（WT-HFD）以及(IV) 高脂饲料处理的肝脏特异性MyD88敲除小鼠（LKO-HFD）。