Differentially expressed adipocyte-associated genes in Bone-marrow Mesenchymal stem cells under normal and hypoxic conditions

To identify proteins involved in the regulation of adipogenic differentiation under hypoxic conditions, an RT2 Profiler PCR array was used to screen a panel of 84 genes associated with human adipogenesis in Bone-marrow Mesenchymal stem cells under normal and hypoxic conditions. Bone-marrow Mesenchymal stem cells were put in hypoxic chamber, set at an oxygen concentration of 0.2%. Cells under normoxia are considered as a control.

为鉴定低氧条件下参与成脂分化调控的蛋白质，本研究采用RT2 基因表达谱PCR芯片（RT2 Profiler PCR array），在常氧与低氧培养条件下的骨髓间充质干细胞（Bone-marrow Mesenchymal stem cells）中，筛选一组共84个人类成脂相关基因。将骨髓间充质干细胞置于氧浓度设定为0.2%的低氧培养箱中培养，以常氧培养的细胞作为对照。