Aroma Profile and Antioxidant Activity of Sweet Basil Aqueous Extracts Affect by Light Modification

This study examines the action of light modifications by different shade nets (pearl, red and blue color nets with a shade index of 50%) in relation to unshaded plants from the open field on the relative effects on changing basil aroma profile and antioxidant activity. The most abundant components were linalool, 1,8-cineole and myrcene. Aqueous extracts were isolated from basil leaves using maceration and ultrasound-assisted extraction. Yield of total extractive matters depends on both the extraction technique applied and the growing conditions used. The highest yield of total extractive matter (30.50 g/100g) was obtained by maceration from open field-grown basil. The highest content of total phenols (106.52 mg GAE/g), flavonoids (19.74 mg RE/g) and antioxidant activity (EC50 - 7 µg/mL) was found under a red net. The isolated water extracts are a source of natural antioxidants with potential application in the food and pharmaceutical industries.

本研究以露地无遮阳栽培的罗勒植株为对照，探究不同遮阳网（珍珠色、红色、蓝色遮阳网，遮阳率均为50%）所营造的光环境变化对罗勒香气成分谱与抗氧化活性的相对影响。研究检测到罗勒中含量最高的香气成分为芳樟醇（linalool）、1,8-桉叶素（1,8-cineole）与月桂烯（myrcene）。本研究采用浸渍法（maceration）与超声辅助提取法（ultrasound-assisted extraction）从罗勒叶片中提取水提物。总浸出物得率同时受所采用的提取技术与栽培条件的影响：露地栽培罗勒经浸渍法提取所得的总浸出物得率最高，达30.50 g/100g。红色遮阳网覆盖下的罗勒，其总酚（total phenols）含量（106.52 mg 没食子酸当量（gallic acid equivalent，GAE）/g）、黄酮类（flavonoids）物质含量（19.74 mg 芦丁当量（rutin equivalent，RE）/g）与抗氧化活性（半最大效应浓度EC50为7 µg/mL）均为最高。所提取的水提物为天然抗氧化剂的优质来源，在食品与医药工业中具备潜在应用价值。