Identification of Pol II(G) target genes in mouse liver [ChIP-seq]

To investigate the biological role of Pol II(G), we identified Pol II(G) target genes in normal mouse liver. To identify genes that are directly targeted by Pol II(G) in hepatocytes, we performed ChIP-seq analysis with non-crosslinked chromatin prepared from Gdown1 flox/flox (FF) or Gdown1flox/flox;Alb-Cre (KO) liver at 7W of age. We also analyzed Gdown1 target genes in p53KO hepatocytes. For the negative control, tamoxifen (TAM)-inducible Gdown1flox/floxKO mice carrying both the Alb-CreERT2 transgene and a p53 null mutation was used.

为探究Pol II(G)的生物学功能，我们在正常小鼠肝脏中鉴定了Pol II(G)的靶基因。为鉴定肝细胞中直接受Pol II(G)靶向调控的靶基因，我们对7周龄Gdown1 flox/flox（FF）或Gdown1 flox/flox;Alb-Cre（KO）小鼠肝脏制备的非交联染色质开展了染色质免疫共沉淀测序（Chromatin Immunoprecipitation sequencing，ChIP-seq）分析。我们还在p53敲除（p53KO）肝细胞中分析了Gdown1的靶基因。本研究使用同时携带Alb-CreERT2转基因与p53纯合缺失突变的他莫昔芬（Tamoxifen，TAM）诱导型Gdown1 flox/flox KO小鼠作为阴性对照。