Cell atlas of mouse colon tissues of liver cirrhosis mouse. Cell atlas of mouse colon tissues of liver cirrhosis mouse

Liver disease alters the gut microenvironment by liver-gut axis. To investigate the composition and transcriptome changes of various intestinal cell populations in liver cirrhosis, we delineated a single-cell atlas of the colon from mice treated CCl4 for 6 weeks. Overall design: 6-week-old SPF mice were treated with CCl4 for 6 weeks, and the mild-colon tissues were harvested for single-cell RNA sequencing.

肝病可通过肝-肠轴（liver-gut axis）改变肠道微环境。为探究肝硬化状态下各类肠道细胞群的组成及转录组变化，我们对经四氯化碳（CCl4）处理6周的小鼠结肠绘制了单细胞图谱。
实验整体设计：6周龄的无特定病原体（Specific Pathogen Free, SPF）小鼠经CCl4处理6周后，采集其轻度病变结肠组织用于单细胞RNA测序（single-cell RNA sequencing）。