Monitoring of the microbiota in the application of a nZVI-based nanoremediation strategy in heavy metal contaminated soil

The project addressed the efficiency of a nanoremediation strategy using zero-valent iron nanoparticles (nZVI), in a case of co-mingled heavy metals (HM) pollution (Pb, Cd and Zn) and a highly damaged soil environment. We applied a combined set of physical-chemical, toxicological and molecular analyses to assess the effectiveness and ecosafety of using nZVI (5% w/w) for environmental restoration. Theses analyses include the taxonomic analysis of the soil microbial community structure by NGS in nZVI-treated and non-treated samples at 0, 15 and 120 days of treatment.

本研究针对铅Pb、镉Cd与锌Zn构成的复合重金属（heavy metals，HM）污染且土壤环境遭受严重破坏的场景，评估了零价铁纳米颗粒（zero-valent iron nanoparticles，nZVI）纳米修复策略的应用效能。本研究采用理化分析、毒理学分析与分子生物学分析相结合的综合手段，对5%质量比（w/w）的nZVI用于环境修复的有效性与生态安全性进行评价。上述分析涵盖通过下一代测序技术（Next Generation Sequencing，NGS），对经nZVI处理与未处理的土壤样本在修复处理第0天、15天及120天时的土壤微生物群落结构开展分类学分析。